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1. we can test the efficacy of the crafted vaccine on to the infected organ (since you are planning on using the infected organ to be useable as a crafting material for the vaccine) of course the result would be dependent on the level of first aid and number of successful attempt in creating an vaccine (up to be decided).
2. or we can test the crafted vaccine to our sample blood in syringe by looking and using the microscope of course the result would be dependent on the level of first aid
3. the vaccine that was crafted should have side effects like flu, cold, or loss of appetite etc.
4. I know this is too much but we can test the vaccine into the zombies itself (the lower the efficacy the greater the side effects like becoming tough etc.)