Contains:

Moonlight Blessing: Gain or Strengthen a Vampire Power (Ultimate Ability Slot)

Contains:

Fresh Blood (Level Up or Gain Currency)

Ruby Gem (Used for Upgrading Main and Secondary Weapons after a run or increasing Companion Affinity)

Contains:

Lillian NPC (Affinity Gives: Extra Garlic Pots, Advanced Garlic Chance, Discount on Items)

Sells:

Garlic (Food that grants protective shield against Vampire attacks)
Advanced Garlic (Food that increases the players max health)

Contains:

Fresh Blood (Level Up or Gain Currency)

Secondary Fresh Blood (Level Up Only) that spawns after the first one has been Absorbed

Contains:

Enhancement Circle (Upgrade an Artifact)

Contains:

Mini Boss Room

Drops a chest with Random Artifact once the Boss dies.

Contains:

Fresh Blood (Level Up or Gain Currency)

Blood Ore through the room that directly drops Fresh Blood (Level Up or Gain Currency) when broken

Contains:

Blood Grail (Increase the Players Maximum Health)

Contains:

Nemera NPC (Affinity Gives: Blood Donation & Transfusion Effects Increase, Scales per Affinity)

Abilities:

Tranfusion Gains HP
Donation Sacrifices HP for Fresh Blood (Level Up or Gain Currency)

Note: Donation sacrifices the same amount of HP that Tranfusion heals. If done in this order they can be used to generate a free Fresh Blood (Level Up or Gain Currency)

Contains:

Fresh Blood (Level Up or Gain Currency)

Silver Currency (Used for: Purchase ability upgrades after a run)

Contains:

Evalaine NPC (Affinity Gives: Reroll Count +1, Sale Price +5% Scaling per level)

Buys your Artifacts for Fresh Blood (Gain Currency Only)

Sells 3 Random Artifacts

Contains: Main Boss

4 Fresh Blood pedestals that protect the bosses coffin and spawn waves when opened.

Once the Main Boss is defeated, spawns Portal (Awards a Legacy passive bonus) then teleport the player to the Rest Zone

Rest Zone Contains: Seraph NPC (Affinity Gives: Healing Amount Increase, Scales per level)

Ability: Heals the player after Main Boss encounter based on affinity.