1. You can shoot the purple level start crystal before it makes full shape.
2. You can activate a speedrunning timer in the settings.
3. You can change enemy colors in the settings.
4. You can now bind abilities to certain keys so you don't have to toggle them with "Q".
5. You don't have to collect any of the shields at the fountains.

That's all I got.
Sorry if this didn't help, your welcome if it did.
I will most likely update this list in the future.