added for trade ^^

+rep sign , add me pls )

Later in Despicable Me 2, we see Dr. Nefario cure the Minions using an antidote mixed with the Jelly from earlier in the film. The PX-41 Mutagen, and its respective cure are solid evidence that the Minions are not entirely immortal and possess a severe and potentially lethal vulnerability to biological and chemical attacks. While some may argue that the PX-41 Mutagen and its antidote did not kill the Minions, a disease or toxic gas could prove fatal showing they can be drastically effected by chemical and biological substances.

Evidence No. 3: Stalin and Hitler both attracted Minions in large quantities to both sides of the Eastern Front, which would result in the gruesome Minion Civil War.

Proof:
Stalin and Hitler were both evil tyrants who would be seen as nothing more than soulless monsters to any human, but for the Minions, saying they were gods amongst Minion would be an understatement. With Stalin's gulags and Hitler's concentration camps, the two of them would be the greatest villians Minion kind had witnessed. With World War 2 beginning in the 1930s, the Minions suffered a brutal split in their society and culture, now voluntarily choosing sides of the Second World War between the two tyrants of the Eastern Front. Because of this, the Minions would engage in a brutal war to end the disagreement of whether Stalin or Hitler would be more deserving of Minion service and worship.

Given their vulnerability to chemical agents, both Human and Minion soldiers of the Eastern Front would release chemical agents such as Chlorine, Phosgene and Mustard Gas across the front lines to attack the other side's Minion forces. Given the sheer bloodiness of the Eastern Front, its likely that the majority of the Minions would be killed in the Second World War. Only a few isolated pockets of Minions would survive following the Second World War, with a primary example being the Minions in the ice cave, having emerged following the World Wars with no knowledge of Stalin Or Hitler and the near extinction of their race.

Evidence of a Minion Civil War occurring and being possible can be found at the end of Despicable Me 3, in which a sizable chunk of Gru's Minions defect to Dru, having deemed Dru more evil and more worth their service, along with the remaining chunk of Minions choosing to remain with Gru.

Closure:

It is unclear what happens after Gru gives chase to Dru at the end of Despicable Me 3, however it can be safely assumed that Despicable Me 4 will cover the complete extinction of the last remaining Minions as they battle it out through various means of chemical warfare over whether Gru or Dru is more evil.